How do I complete the initial focus set up on a stereo zoom microscope?
1) Center your specimen on the stage for observation.
2) Adjust your lighting so that the specimen is properly illuminated on the stage.
3) Set your adjustable eyepiece(s) to 0. Your dot (marking) on the eyepiece collar should match up with the 0.
4) Set your zoom to the highest power.
5) Use the focus block to bring your specimen into focus.
6) Using your right eye only, look through the eyepiece on the right side and focus it so that the image is at it’s sharpest.
7) Using your left eye only, look through the eyepiece on the left side and focus it so that the image is at it’s sharpest.
8) Rotate your zoom knob to the lowest power. Your microscope should now be calibrated so that your specimen is in focus throughout the entire zoom range.
The highest quality optics, durability of mechanical components, and customer service have Nikon ranked as our number one choice.
Olympus comes in a strong second as a company known for performance and ergonomics on it’s microscopes. You’ll have to exercise patience when dealing with Olympus though.
Leica ranks third in our opinion. The once great German optical company seems to have moved manufacturing outside of Europe on some microscopes and the quality seems to be falling off based on the models we’ve evaluated.
The only microscope company that manufacturers it’s microscopes completely in Japan as of this writing. (Nikon and Olympus, both Japanese companies have opted to manufacture throughout Asia at this point) Meiji is not a well known name, but based on price and quality, they’re definitely in the top 5.
Zeiss still makes the world’s best surgery microscopes. However their upright compound microscopes don’t seem to be in the same class that they once were. This quality fall off and the price have Zeiss microscopes down in the 5th position. Customer service is also a challenge.
Labomed is the relative “new kid on the block” and have a surprisingly well developed proprietary line of microscopes. This company out of India has good quality optics and a very sleek design. Support is good, and the best part is the pricing. We really like these microscopes as our choice for second tier microscopes outside of the top 5.
Accuscope has a rapidly growing line of new microscope models. This NY based company has the majority of their line manufactured in China. The price is right on these microscopes, they just have to get the quality control issues hammered out. Their customer service is top notch and their microscopes do come with the standard 5 year warranty.
A straight line micrometer (sometimes referred to as a reticle) is a measuring device installed in the eyepiece of a microscope. The ocular micrometer consists of a straight line numbered 1 through 10 divided into one hundred divisions. The metric between each division changes based on the different powers of magnification used.
An example of how to calibrate an eyepiece micrometer can be found at the following link: Calibrating an Eyepiece Micrometer